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negative control nc sirna  (Obio Technology Corp Ltd)

 
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    Obio Technology Corp Ltd negative control nc sirna
    Negative Control Nc Sirna, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/negative control nc sirna/product/Obio Technology Corp Ltd
    Average 86 stars, based on 1 article reviews
    negative control nc sirna - by Bioz Stars, 2026-05
    86/100 stars

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    SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after <t>siRNA-mediated</t> knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.
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    SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after <t>siRNA-mediated</t> knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.
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    SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.

    Journal: Nucleic Acids Research

    Article Title: In situ tracking of glycoRNAs on single-cell surface to reveal RNA heterogeneity and transport mechanism

    doi: 10.1093/nar/gkag362

    Figure Lengend Snippet: SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.

    Article Snippet: siRNAs targeting human VTI1B, TSNARE1, and GAPDH, as well as a non-targeting negative control siRNA, were synthesized by Sangon Biotech (Shanghai, China).

    Techniques: Knockdown, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Labeling